Journal: PLoS ONE

Article Title: The Diagnostic and Prognostic Accuracy of Five Markers of Serious Bacterial Infection in Malawian Children with Signs of Severe Infection

doi: 10.1371/journal.pone.0006621

Figure Lengend Snippet: Comparison of median CRP, PCT, s-TREM-1, CD163 and HMGB1 values in different groups stratified by HIV status.

Article Snippet: TREM-1, CD163 and HMGB1 were measured using a commercially available sandwich enzyme immunoassays according to the manufacturer's instructions (TREM-1: Quantikine, R&D Systems, Minneapolis, USA, CD163: Bachem (UK), St Helens, Merseyside, UK, and HMGB1: Shino-test, Kanagawa, Japan).

Techniques: Comparison

Journal: PLoS ONE

Article Title: The Diagnostic and Prognostic Accuracy of Five Markers of Serious Bacterial Infection in Malawian Children with Signs of Severe Infection

doi: 10.1371/journal.pone.0006621

Figure Lengend Snippet: Correlation between markers of infection and pro-and anti- inflammatory cytokines (Spearman's correlation coefficient. NS = non-significant).

Article Snippet: TREM-1, CD163 and HMGB1 were measured using a commercially available sandwich enzyme immunoassays according to the manufacturer's instructions (TREM-1: Quantikine, R&D Systems, Minneapolis, USA, CD163: Bachem (UK), St Helens, Merseyside, UK, and HMGB1: Shino-test, Kanagawa, Japan).

Techniques: Infection

Journal: PLoS ONE

Article Title: The Diagnostic and Prognostic Accuracy of Five Markers of Serious Bacterial Infection in Malawian Children with Signs of Severe Infection

doi: 10.1371/journal.pone.0006621

Figure Lengend Snippet: ROC curve of CRP, PCT, s-TREM-1, CD163 and HMGB1 as markers of SBI.

Article Snippet: TREM-1, CD163 and HMGB1 were measured using a commercially available sandwich enzyme immunoassays according to the manufacturer's instructions (TREM-1: Quantikine, R&D Systems, Minneapolis, USA, CD163: Bachem (UK), St Helens, Merseyside, UK, and HMGB1: Shino-test, Kanagawa, Japan).

Techniques:

Journal: PLoS ONE

Article Title: The Diagnostic and Prognostic Accuracy of Five Markers of Serious Bacterial Infection in Malawian Children with Signs of Severe Infection

doi: 10.1371/journal.pone.0006621

Figure Lengend Snippet: ROC plot of CRP, PCT, s-TREM-1, CD163 and HMGB1 as predictors of mortality.

Article Snippet: TREM-1, CD163 and HMGB1 were measured using a commercially available sandwich enzyme immunoassays according to the manufacturer's instructions (TREM-1: Quantikine, R&D Systems, Minneapolis, USA, CD163: Bachem (UK), St Helens, Merseyside, UK, and HMGB1: Shino-test, Kanagawa, Japan).

Techniques:

Journal: Journal of neurovirology

Article Title: Elevated Cerebrospinal Fluid Galectin-9 is Associated with Central Nervous System Immune Activation and Poor Cognitive Performance in Older HIV-infected Individuals

doi: 10.1007/s13365-018-0696-3

Figure Lengend Snippet: CSF Gal-9 associations with CSF markers of CNS immune activation and neuronal injury

Article Snippet: Soluble markers were determined by the following quantitative kits: Human Galectin-9 Quantikine ELISA kit (R&D systems, MN, USA), Soluble CD163 ELISA (Trillium Diagnostics, LLC, ME, USA), Neopterin competitive enzyme immunoassay (ALPCO, NH, USA), and NF-Light (Neurofilament light) ELISA (UmanDiagnostics, Sweden).

Techniques: Activation Assay, Marker

Journal: PLoS ONE

Article Title: Heme oxygenase-1 as an important predictor of the severity of COVID-19

doi: 10.1371/journal.pone.0273500

Figure Lengend Snippet: (A) HO-1 according to disease severity; (B) sCD163 according to disease severity; (C) Comparison of the prediction of severity between HO-1 (red line) and sCD163 (blue line). (A, B) Both serum HO-1 and sCD163 indicated high levels according to the disease severity of COVID-19. The center bold line is the median value; the bottoms and tops of the boxes represent the 25th and 75th percentiles, respectively; and the whiskers are 95% confidence intervals. (C) Comparing the prediction of disease severity between HO-1 and sCD163 (severe vs. moderate and mild), serum HO-1 shows a higher AUC (0.875) than sCD163 (0.733).

Article Snippet: Serum HO-1 levels were measured on admission using the ImmunoSet ™ HO-1 enzyme-linked immunosorbent assay (ELISA) development set (Enzo, Farmingdale, NY, USA) and sCD163 levels were measured using Quantikine ELISA Human CD163 Immunoassay (R&D Systems, Minneapolis, MN, USA) according to the manufacturers’ instructions.

Techniques:

Journal: PLoS ONE

Article Title: Heme oxygenase-1 as an important predictor of the severity of COVID-19

doi: 10.1371/journal.pone.0273500

Figure Lengend Snippet: The receiver operating characteristic (ROC) curve analyses for serum HO-1 and sCD163 levels to predict treatment outcomes including ICU admission and mechanical ventilation or ECMO support. The area under the ROC curve (AUC) for predicting ICU admission was 0.816 for serum HO-1 and 0.743 for sCD163 (A). The AUC for predicting mechanical ventilation support is 0.827 for serum HO-1 and 0.696 for sCD163 (B). The AUCs for predicting ECMO support are 0.790 and 0.598, respectively (C).

Article Snippet: Serum HO-1 levels were measured on admission using the ImmunoSet ™ HO-1 enzyme-linked immunosorbent assay (ELISA) development set (Enzo, Farmingdale, NY, USA) and sCD163 levels were measured using Quantikine ELISA Human CD163 Immunoassay (R&D Systems, Minneapolis, MN, USA) according to the manufacturers’ instructions.

Techniques:

Journal: PLoS ONE

Article Title: Heme oxygenase-1 as an important predictor of the severity of COVID-19

doi: 10.1371/journal.pone.0273500

Figure Lengend Snippet: (A) Serum HO-1 and GGO and consolidation score for predicting intensive care unit (ICU) admission: the area under the receiver operating characteristic curve (AUC): 0.915 (P < 0.001); (B) Serum HO-1 and GGO and consolidation score for predicting mechanical ventilation: AUC 0.919 (P < 0.001); (C) Serum HO-1, sex, and GGO and consolidation score for predicting ICU admission: AUC 0.916 (P < 0.001); (D) Serum HO-1, sex, and GGO and consolidation score for predicting mechanical ventilation: AUC 0.920 (P < 0.001). (A, C) Analysis of the prediction of ICU admission and mechanical ventilation support using composite parameters including serum HO-1, sex, and the GGO and consolidation score show that the combination has a higher the AUC than the AUCs of a single predictor (only HO-1) or the combination of two (serum HO-1 and GGO and consolidation score). (B, D) The composite parameters including serum HO-1, sex, and the GGO and consolidation score calculated for prediction of mechanical ventilation support shows a higher AUC than AUCs of a single predictor or a combination of two.

Article Snippet: Serum HO-1 levels were measured on admission using the ImmunoSet ™ HO-1 enzyme-linked immunosorbent assay (ELISA) development set (Enzo, Farmingdale, NY, USA) and sCD163 levels were measured using Quantikine ELISA Human CD163 Immunoassay (R&D Systems, Minneapolis, MN, USA) according to the manufacturers’ instructions.

Techniques:

Journal: PLoS ONE

Article Title: Heme oxygenase-1 as an important predictor of the severity of COVID-19

doi: 10.1371/journal.pone.0273500

Figure Lengend Snippet: We did follow up the HRCT scores in 31 patients and divided these cases into the low baseline HO-1 group (N = 16) and the high baseline HO-1 group (N = 15). We found that in the former group, the GGO and consolidation score were significantly decreased (A), while in the latter group, it was persistently high (B).

Article Snippet: Serum HO-1 levels were measured on admission using the ImmunoSet ™ HO-1 enzyme-linked immunosorbent assay (ELISA) development set (Enzo, Farmingdale, NY, USA) and sCD163 levels were measured using Quantikine ELISA Human CD163 Immunoassay (R&D Systems, Minneapolis, MN, USA) according to the manufacturers’ instructions.

Techniques:

Journal: Nature Communications

Article Title: Prolonged experimental CD4 + T-cell depletion does not cause disease progression in SIV-infected African green monkeys

doi: 10.1038/s41467-023-36379-2

Figure Lengend Snippet: Assessment of gut integrity was performed by monitoring a marker of gut damage in plasma, intestinal fatty acid binding protein (I-FABP) ( a ), and the expression of a tight-junction protein, claudin-3 ( b – f ), by immunohistochemistry (IHC) in CD4 + -cell-depleted AGMs (blue) and controls (yellow). Assessment of microbial translocation was performed by monitoring two soluble markers of monocyte activation, soluble CD163 (sCD163) ( g ) and soluble CD14 (sCD14) ( q ), that are associated with microbial translocation and disease progression. Microbial translocation was also monitored by quantifying the expression of lipopolysaccharide (LPS, brown) in sLNs ( h – p ) and in colon tissues ( r – v ), by IHC. For IHC, images are representative of n = 6 animals in each group, with at least 20 images taken per animal for colon tissues and at least 9 images for sLNs. For all images, the scale bar represents 100 μm. Data presented are means and standard deviations of the means for ELISA ( a , g , q ), and means and standard deviations for IHC ( b , h , r ) ( n = 6 AGMs for CD4 + -cell-depleted AGMs and controls, except for ( b ) and ( r ) for which n = 5 AGMs for controls at baseline). Data in ( b ), ( h ) and ( r ) were analyzed with unpaired, two-sided, nonparametric Mann–Whitney tests, followed by Holm-Šídák’s correction for multiple comparisons. AGMs African Green Monkeys, sLNs superficial lymph nodes, LPS lipopolysaccharide, ns not significant, NX necropsy, PRE preinfection.

Article Snippet: Different soluble markers were quantified in plasma of AGMs, using immuno-assays: sCD14 (Quantikine Human sCD14 Immunoassay, R&D Systems, Minneapolis, MN, USA), sCD163 (Macro163, IQProducts, Netherlands), I-FABP (Monkey I-FABP/FABP2 ELISA Kit, MyBioSource, San Diego, CA, USA) and CRP (Monkey CRP ELISA kit, Life Diagnostics, PA, USA).

Techniques: Marker, Binding Assay, Expressing, Immunohistochemistry, Translocation Assay, Activation Assay, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY